在位及异位子宫内膜组织建立子宫内膜异位症动物模型的研究

Human uterine endometrium and endometiotic tissue transplanted into nude mice to establish an animal model of endometriosis

目的建立子宫内膜异位症的种植动物模型,了解正常在位子宫内膜、腺肌症的子宫内膜及卵巢巧克力囊肿内壁组织在裸鼠体内的生长的差异。方法将正常子宫内膜、子宫腺肌症患者的内膜及卵巢巧克力囊肿内壁分别注射到裸鼠腹腔内,观察生长情况,留取标本行病理及免疫组化检查。结果3种种植物中以卵巢巧克力囊肿内壁最易种植成功,其次是子宫腺肌症患者的内膜,正常子宫内膜种植成功率低于前两者。免疫组化结果提示3组种植成功的腺上皮上均有雌激素受体表达,孕激素受体弱表达或不明显,血管上皮生长因子表达明显。结论腺肌症的子宫内膜及卵巢巧克力囊肿内壁组织在裸鼠体内更易生长,与正常内膜有差异;裸鼠体内种植腺体上皮及基质均见明显血管上皮生长因子表达。裸鼠子宫内膜异位症的动物模型成功建立,为研究子宫内膜异位症的发病机理和药物治疗提供了实验动物模型。

Objective To establish the implanted animal model of human endometriosis.Methods The eutopic endometrium of normal women and patients of endometrioma,and the inner tissue of the cystis of ovarian endometriosis were implanted into the abdomen of nude mice respectively.Its growing condition was observed and the specimen was saved for pathology and immunohistochemistry examination.Results Of the three tissues,the eutopic endometrium of patients of endometrioma grew most easily.The second wes the inner tissue of the cystis of ovarian endometriosis.The successful implantation rate of the the eutopic endometrium of normal women into the nude mice was the lowerest.Immunohistochemistry examination showed that oestrogen receptors were expressed in the glandular epithelia and in stromal cells of endometriotic lesions of the three groups,whereas progesterone receptors were weakly expressed or not expressed.The vascular endothelial growth factor（VEGF） was clearly observed in both the glandular epithelia and stromal cells of grafts of the three groups.Conclusion The eutopic endometrium of patients with endometrioma and the inner tissue of the cystis of ovarian endometriosis are different from the eutopic endometrium of normal women.They could be more successfully transplanted into nude mice.The successful establishment of the animal model of endometriosis can provide an ideal model for the further investigation of the mechanisms and medical therapy of endometriosis.