摘要
目的:筛选人肺腺癌细胞中全反式维甲酸(altrans-retinoicacid,ATRA)激活表达的基因。方法:以我室构建的ATRA诱导前后人肺腺癌细胞系GLC-82的cDNA削减文库各克隆作为探针,与ATRA处理前后GLC-82的cDNA文库Lib.GLC-82,Lib,GLC-82.RA1,Lib.GLC-82.RA4进行Southern杂交,以筛选在ATRA处理后GLC-82cDNA文库中特异存在的cDNA片段。以筛选出的特异存在于ATRA处理后GLC-82cDNA文库中的cDNA片段作为探针,与胎儿各组织总RNA进行Norther杂交,以检测其在胎儿各组织中的表达情况。结果:其中一克隆RA97特异存在于ATRA诱导1天cDNA文库,核酸序列同源分析表明,RA97与人22号染色体长臂13区-PAC克隆PAC408N23同源性很强,其中一段(约600bp)与PAC408N23几乎完全同源;此片段与可能的肿瘤抑制基因SNC6同源性也相当强,其中一段(约350bp)与SNC6几乎完全同源。RA97在多种胎儿组织中均有表达,转录本有两个,分别为4.3kb和4.8kb。结论:获得一特异存在与ATRA诱导1天GL?
Objective: To screen the cDNA subtractive libraries of human lung adenocarcinoma cell line GLC-82 treated by all-trans retinoic acid for different durations. Methods: A cDNA fragment RA97 was obtained from the library of one day treatment. Results: Northern blot results showed that RA97 was expressed in different fetal tissues with two transcrips of 4.5kb and 5.1 kb respectively. Homology analysis showed that RA97 was highly homologous with PAC408N23 at human 22q13 and candidate tumor suppressor gene SNC6. Aproximately 600bp and 350bp DNA sequences of RA97 were identical with corresponding regions of PAC408N23 and SNC6 respectively. Conclusion: These results indicate that RA97 probably has some relationship with tumorogenesis.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
1999年第2期87-90,共4页
Chinese Journal of Clinical Oncology
基金
攀登计划项目
"863"资助