p16,Cyclin D1在胃癌发生过程中的表达

Expression of p16 and Cyclin D1 in Carcinogenesis of Stomach

目的：研究ｐ１６，ＣｙｃｌｉｎＤ１在胃癌发生过程中的表达。方法：采用免疫组化ＡＢＣ法，检测胃癌、不典型增生、萎缩性胃炎及正常胃粘膜组织ｐ１６和ＣｙｃｌｉｎＤ１的表达。结果：ｐ１６蛋白在正常胃粘膜表达率最高（８６７％，１３／１５），并随病变进展（浅表性胃炎－萎缩性胃炎－不典型增生－腺癌），表达率呈下降趋势（分别为８５％；７８６％；３１８％；４８３％），其中不典型增生、腺癌组与正常组比较差异显著（Ｐ＜００５）。ＣｙｃｌｉｎＤ１过度表达在萎缩性胃炎即已出现，并随病变发展表达率升高，萎缩性胃炎、不典型增生、腺癌的ＣｙｃｌｉｎＤ１蛋白表达率分别为１７９％（５／２８）；３６４％（８／２２）；５３４％（３１／５８），其中腺癌与萎缩性胃炎差异显著（Ｐ＜００５）。相关性分析显示，大多数受检胃癌组织中，ｐ１６与ＣｙｃｌｉｎＤ１呈反向表达。结论：ｐ１６和ＣｙｃｌｉｎＤ１在胃上皮癌变过程中起着重要作用，其在胃癌中的反向表达趋势提示两者可能存在相互抑制机制。

p16 and Cyclin D1 expressions were determined in the specimens of gastric carcinoma, atypical hyperplasia, atrophic gastritis, superficial gastritis and normal gastric mucosa by using immunohistochemical method ABC).The results showed that positive immunostaining rate for p16 protein was the highest in normal gastric mucosa and decreased with progression from superficial gastritis to atrophic gastritis to atypical hyperplasia and to adenocarcinoma (85%, 78.6%, 31.8%, 48.3%respectively). Positive immunostaining of cyclin D1 could be observed in atrophic gastritis with the lesions progressing from atrophic gastitis to atypial hyperplasia and to adenocarcinoma, the expression rates increased in order 17.9%, 36.4%, 53.4%respectively. There was a significant difference between adenocarcinoma and atrophic gastritis group (P< 0.05). An interesting observation was that inverse expression between p16 and Cyclin D1 was shown in most of the gastric cancer tested. The results suggest that p16 and Cyclin D1 play an important role in gastric carcinogenesis, and that the inverse expression between p16 and Cyclin D1 suggests antagonism between the two.