抑凋谢基因bcl-2蛋白在大鼠实验性肝癌发病过程中的表达

The expression of bcl 2 protein during 3′ MeDAB induced hepatocarcinogenesis of rats

目的动态观察大鼠实验性肝癌形成过程中抑凋谢基因ｂｃｌ－２蛋白表达与γ－谷氨酰转肽酶（γ－ＧＴ）活性和谷胱甘肽硫转移酶（ＧＳＴ）活性改变的关系以及刺五加多糖的抑癌作用。方法用ｂｃｌ－２单抗进行免疫组化ＳＡＢＣ检测，用酶学动力学方法检测酶活性。结果饲喂致癌剂３－甲氨基偶氮苯（３′－ＭｅＤＡＢ）的早期（１５天），ｂｃｌ－２蛋白表达已明显增高，实验结束时３′－ＭｅＤＡＢ组阳性率为８９．５％（１７／１９），刺五加组为６４．７％（１１／１７）。ｂｃｌ－２的过量表达与肝组织中γ－ＧＴ活性升高和ＧＳＴ活性降低相一致。结论ｂｃｌ－２蛋白过度表达出现，对实验性肝癌的早期阶段，很可能在肿瘤的发生发展中起着促进作用。

Objective To study observed the expression of bcl 2,γ glutamyl transpeptidase (γ GT) activities and glutathione stransferase (GST) activities during 3′ MeDAB induced hepatocarcinogenesis of rats,and to demonstrate the distinct inhibitory effect of Acanthopanax senticosus to neoplastic proliferation.Methods Immunohistologic staining for MoAb bcl 2 was performed on liver tissve sectious from 3′ MeDAB treated rats.The γ GY and GST activity was determined by enzymatic dynamic method.Results Bcl 2 was expressed in 89.5%(17/19) of the 3′ MeDAB treated rats and in 64.7%(11/17) of the Acanthopanax senticosus treated rats.Bcl 2 over expression was an early event in hepatocarcinogenesis followed by increase of r GT activity and decrease of GST activity.Conclusions The data indicate that the bcl 2 expression is characteristic of the early phase of hepatocarcinogenesis.Acanthopanax senticosus have distinct inhibitery effect on neoplastic proliferation.