胃蛋白酶胰蛋白酶水解酪蛋白的研究

Research on the Hydrolytic Course from Casein with Pepsin and Trypsin

主要研究胃蛋白酶、胰蛋白酶水解酪蛋白的过程。首先通过正交试验研究了胃蛋白和胰蛋白酶水解酪蛋白的最佳条件,分别为酶浓度0.15%、温度55℃、pH7.5、底物浓度6%和酶浓度1%、温度55℃、pH=1.6、底物浓度1%。然后对于胃蛋白酶胰蛋白酶组合水解进行研究,最后通过层析分离和液相色谱初步验证了水解产物含有β-啡肽-7等生物活性多肽。

The focus of the experiment is reseach on the hydrolytic course from casein with pepsin and trypsin. Firstly through orthogonals experiments design to carry on to the best enzymolysis further discusses and obtains the best test condition according to the test result union actual factor,enzyme concentration 1.5 %,temprature 55 ℃,pH 7.5,substrate concentration and enzyme concentration 1 %,temprature 55 ℃,pH 1.6,substrate concentration 1 %. Reseach on the hydrolytic course from trypsin-pepsin, Finally the experiment verify that hydrolysates containing β-CM-7 biological activity peptides by SephadexG-15 and RP-HPLC.