AOT/异辛烷反胶束体系制备大豆蛋白产品研究

The Analysis Research on Product Component of Soybean Protein Extracted Using Reversed Micelles

采用不连续SDS-聚丙烯酰胺凝胶电泳分析、氨基酸分析和差示扫描量热分析等多种方法对丁二酸二异辛酯磺酸钠(AOT)/异辛烷反胶束萃取的产品大豆蛋白和传统的碱溶酸沉法生产的大豆分离蛋白(SPI)进行分析比较。结果发现反胶束萃取的大豆蛋白中β-折叠和α-螺旋含量比大豆分离蛋白少。但氨基酸种类齐全,含有8种必需氨基酸,且两种产品蛋白中氨基酸所占蛋白的比例大致相似,反胶束萃取的蛋白中天冬氨酸、谷氨酸、精氨酸和色氨酸的含量比碱溶酸沉法生产的大豆分离蛋白高。反胶束萃取的大豆蛋白结构相对比较稳定,变性温度为77℃左右,变性程度比碱溶酸沉法生产的蛋白小。

The differences of the component of soybean protein extracted by bis-（2-ethylhexyl）sulfosuccinate （AOT）/iso-octane reverse micelle system and prepared by alkali-solution and acid-isolation was investigated by the discontinuities SDS-PAGE electrophoresis analysis, amino acid analysis and the differential scanning calorimetry analysis. It was found that the contents of β-sheet and α-helix of the protein extracted by reverse micelles were less than them of SPI. It contains of 8 kinds amino acid, and that the proportion of each amino acid in the soybean protein extracted by reverse micelles was about the same with that of SPI. The contents of aspartic acid, glutamic acid, arginine and tryptophan in the protein extracted by reverse micelles were higher than SPI. The structure of soybean protein extracted by reverse micelles relatively stabilize. The temperature of denaturalization was about 77 ℃, and The grade of denaturalization was smaller than SPI.