栝楼愈伤组织和块根中天花粉蛋白相对含量的比较研究

Comparison of the relative content of trichosanthin between root and callus derived from Trichosanthes kirilowii

目的：分析离体培养的栝楼叶片愈伤组织中天花粉蛋白（ＴＣＳ）的相对含量，并与栝楼块根进行比较。方法：采用丙酮沉淀方法，获得栝楼叶片愈伤和块根的蛋白提取物。应用免疫扩散反应、ＳＤＳＰＡＧＥ蛋白质凝胶电泳和电泳图谱的薄层扫描技术进行检测分析。结果：栝楼愈伤组织提取物中ＴＣＳ的相对含量最高，占被分析的４种总蛋白量的４４２２％。但其ＴＣＳ的绝对含量明显地低于块根中的含量。结论：栝楼块根中ＴＣＳ的绝对含量高于栝楼叶片愈伤组织，大约是愈伤组织的２６６倍。

OBJECTIVE:The relative content of trichosanthin (TCS) of the calli induced from the leaves of Trichosanthes kirilowii Maxim. was measured and a comparison between the calli and the root was made. METHODS:TCS was obtained by the fractional precipitate with acetone from the homogenate of the root or the calli. To examine and measure TCS, several methods, such as immuno precipitation reaction, SDS PAGE and electrophoregram scanning, were usde. RESULTS:The results of immuno precipitation reaction and SDS PAGE showde that TCS existed in the calli and in the root of T.kirilowii Maxim.. It was found that TCS was the richest component in the acetone precipitated crude extract of the calli with a relative content of 44 22% TCS in the extract, though the absolute content of TCS in the calli was less than that in the root. CONCLUSION:Extracting TCS from the calli derived from leaves has not been reported previously. The absolute content of TCS in the root is 2 66 times more than that in the calli.