棉花乙烯受体基因的电子克隆及生物信息学分析

In Silico Cloning and Bioinformatics Analysis on Ethylene Receptor Gene from Cotton

[目的]电子克隆并分析棉花乙烯受体基因(Ethylene Receptor,ETR)。[方法]利用生物信息学方法,以毛果杨的ETR基因家族序列为基础,电子克隆棉花乙烯受体基因开放阅读框,并对序列进行氨基酸组成、功能域、二级结构、疏水性、信号肽及系统进化等预测分析。[结果]通过电子拼接,共获得该基因家族的2个棉花ETR基因GhETR1和GhETR2。2个基因开放阅读框长度分别为2 289 bp和2 226 bp,编码含762和741个氨基酸残基的肽链。经生物信息学分析发现,两基因分属于ETR2和ETR1亚家族。N端有典型的跨膜区,含有ETR蛋白的保守域GAF、H isKA、HATPase-c和REC。GhETR1与毛果杨XP_002315717、XP_002311669相似性较高,GhETR2与毛果杨XP_002299688、XP_002327419相似性较高。[结论]该研究结果为进一步研究棉花GhETR基因在棉纤维发育中的作用奠定了基础。

[Objective]The purpose of this research was to clone and analyze ETR gene of Gossypium.[Method]ETR genes ORF of Gossypium was in silico cloned based on sequences of Populus trichocarpa ETR genes.The sequences were analyzed by bio-informatical tools to predict the aspects,including composition of amino acids,domains,secondary structure,hydrophobicity,signal peptide,and phylogenetic analysis.[Result]The results showed there were at least two ETR genes in Gossypium,named GhETR1 and GhETR2.The two sequences of the genes contained 2 289 bp and 2 226 bp open reading frame（ORF）,encoded 762 and 741 amino acids.They separately belonged to ETR2 and ETR1 subfamily.There were transmembrane domain on N-terminal,GAF,HisKA,HATPase-c and REC conserved domain.GhETR1 was similarity to XP_002315717 and XP_002311669 of Populus trichocarpa,while GhETR2 was similarity to XP_002299688 and XP_002327419.[Conclusion]The result will provide the basis for study about the effect on cotton fiber development.