一株产抗灰霉病菌株的发酵优化研究

Study on the Fermentation Optimization of a Strain Producing the Substance against Botrytis cinerea

[目的]对枯草芽孢杆菌a1发酵条件进行初步优化。[方法]以顺丁烯二酸酐为基础碳源,采用生长速率法测定发酵液对番茄灰霉菌的抑制率。[结果]该菌株较佳发酵条件为:酵母膏1%,含1%顺丁烯二酸酐的PDB培养基培养,温度30℃,接种量1%,装液量50 ml/500 ml,初始pH值5.5,摇床转速150 r/min,发酵时间120 h。[结论]为开发新型微生物源农药奠定基础。

[Objective] The fermentation conditions of Bacillus subtilis a1 were preliminarily optimized.[Method] Using maleic anhydride-based carbon source,the inhibition rate of fermentation liquid against Botrytis cinerea was determined by growth rate method.[Result] The fermentation conditions of the strain were as follows： the PDB medium with 1% maleic anhydride,1% yeast extract,temperature of 30 ℃,1% inoculum,50 ml/500 ml medium volume,initial pH of 5.5,rotating rate of 150 r/min,and culture time of 120 h.[Conclusion] The research laid the foundation for developing new microbial pesticides.