家蝇幼虫离体血细胞感染大肠杆菌后的形态变化

Morphological Changes of the Hemocytes of Musca domestica Larva in vitro Infected by Escherichia coli

[目的]观察大肠杆菌感染对离体家蝇3龄幼虫血细胞形态的影响,了解参与家蝇幼虫细胞免疫反应的细胞类型。[方法]对家蝇3龄幼虫血细胞进行体外培养,并于培养后2、4、6、8 h对血细胞形态进行观察;离体家蝇3龄幼虫血细胞中加入大肠杆菌菌液,观察感染后不同时间血细胞形态的变化。[结果]家蝇三龄幼虫血细胞培养2 h后可区分血细胞的5种类型,培养6 h后血细胞部分贴壁;感染大肠杆菌后浆血胞发生空泡、细胞变形等变化,粒血胞周围见大量细菌聚集,未见原血胞、珠血胞、类绛血胞形态的变化。[结论]浆血胞和粒血胞是家蝇幼虫细胞免疫反应的主要参与者,而原血胞、珠血胞及类绛血胞不参与细胞免疫反应。

[Objective] The research aimed to observe the effects of Escherichia coli infection on the morphology of the hemocytes of the 3rd stage larva of Musca domestica in vitro and understand the hemocytes types that take part in the cell immunity of Musca domestica larva.[Method] The hemocytes of the 3^rd stage larva of Musca domestica were culture in vitro and the hemocyte morphology was observed about 2,4,6 and 8 h after culture in vitro.After Escherichia coli were injected into the hemocytes of the 3^rd stage larva of Musca domestica in vitro,the morphology changes of hemocytes were observed at different time after infection.[Result] The hemocytes of the 3rd stage larva of Musca domestica was divided into five types about 2 h after hemoculture.The hemocytes partly adherence was seen about 6 h after hemoculture.The vacuolation and morpholysis were found in plasmatocytes after being infected by E.coli and a great quantity bacteria were gathered around granulocyte,but the morphology changes of hemocytes were not found in the prohemocyte,sphrulocyte and oenocytoid.[Conclusion] The plasmatocyte and granulocyte are primary participants of the cell immunity of Musca domestica larva,but the prohemocyte,sphrulocyte and oenocytoid do not participate the cell immune reactions.