荆江麻鸭PRL基因内含子1的SNPs检测及其与体尺性状的关联研究

Study on Correlation between Single Nucleotide Polymorphism Detection of Intron 1 of PRL Gene with Body Size Traits in Jingjiang Partridge Duck

[目的]研究荆江麻鸭PRL基因内含子1序列多态性及其对体尺性状的影响。[方法]以96只荆江麻鸭为材料,采用PCR-RFLP方法对PRL基因内含子1序列多态性进行检测,并分析基因多态性与其体尺性状间的关系。[结果]荆江麻鸭PRL基因内含子1具有序列多态性,DraⅠ酶切产生AB和BB 2种基因型,其中BB基因型为优势基因型(91.67%);卡方检验结果表明,群体该位点处于遗传平衡状态(P>0.05);最小二乘分析结果表明,AB基因型个体骨盆宽极显著大于BB基因型个体(P<0.01),BB基因型个体胸围显著高于AB基因型个体(P<0.05)。[结论]初步推断,PRL基因可作为影响荆江麻鸭部分体尺性状的候选基因(标记),选择不同的等位基因可改善不同的体尺性状。

[Objective] The research aimed to study the polymorphism of intron 1 and its effects on prolactin（PRL） gene and body size traits of Jingjiang Partridge Duck.[Method] Taking 96 Jingjiang partridge ducks as materials,the polymorphism of intron 1 of PRL gene was detected by PCR-RFLP method,and the relationship between the gene polymorphism and body size traits was analyzed.[Result] Intron 1 in PRL gene of Jingjiang Partridge Duck had sequence polymorphism.Two kinds of genotypes（AB and BB） were detected by DraⅠ restriction and genotype BB was dominant in population（91.67%）.The results of Chi-square test indicated that this site was in Hardy-Weinberg equilibrium in Jingjiang Duck（P〉0.05）.The results of least square analysis showed that the ducks with AB genotype had larger pelvis breadth（P〈0.01） but smaller chest measurement（P0.05） than those of BB ducks.[Conclusion] It was preliminarily concluded that PRL gene could be regarded as useful candidate gene（marker） for part of body size traits of Jingjiang Partridge Duck and part of body size traits could be improved by allele selection.