DAZ基因外显子与无精子症的关系

THE RELATIONSHIP BETWEEN DAZ EXON AND AZOOSPERMIA

目的探讨Y染色体无精子缺失(DAZ)基因外显子与无精子症的关系。方法应用多重聚合酶链反应(PCR)技术对97例无精子症病人和60例正常生育男性DAZ基因外显子进行检测。结果 97例无精子症病人DAZ基因外显子缺失4例,缺失率4.12%,正常生育男性DAZ外显子未检测到缺失。结论 DAZ基因外显子缺失可导致无精子症;检测不育病人是否有Y染色体微缺失,对于通过辅助生殖技术(ART)将这种基因缺陷遗传给下一代的可能性评估具有重要意义。

Objective To explore the relationship between DAZ exon in Y chromosome and azoospermia.MethodsDAZ exons were detected by polymerase chain reactions（PCR）in 97 patients with azoospermia and 60 normal men.Results Of 97 patients,DAZ exon deletion was found in four（4.12%）.However,DAZ exon deletion was not found in the healthy subjects.Conclusion Deletion of DAZ exon may result in azoospermia.A detection of whether an infertile patient is suffering from Y microdeletion is of great importance to assess the possibility of descending this gene defect to offspring through assisted reproductive technology.