摘要
结合生物学实验教学,对植物细胞有丝分裂实验的取材、预处理条件、染料以及染色方法选择进行了比较研究。结果表明：选用洋葱根尖作为试材;材料预处理选用4℃低温预处理24~36 h;选择Schiff试剂作为染料;用Fuelgen染色法染色为观察植物细胞有丝分裂实验最优化的条件。
The presented article performs a comparison study on the selection of experimental material,the preprocessing condition,the selection of dye,and the selection of dyeing method in the of plant cell mitosis.The results showed that when onion root-tip was selected as the experimental material and preprocessed in 4℃ for 24~36 h,Schiff reagent as the dye,and using Fuelgen dyeing method,the optimal experiment condition for observing the plant cell mitosis was obtained.
出处
《北方园艺》
CAS
北大核心
2010年第13期129-132,共4页
Northern Horticulture
基金
广东省科技计划资助项目(2006B20101011)
湛江市科技攻关资助项目(2008C08022)
关键词
有丝分裂
预处理
条件
优化
mitosis
preprocessing
condition
optimizing
