结缕草种子愈伤组织诱导及植株再生研究

Callus Induction and Plant Regeneration from Seeds of Zoysia japonica

以结缕草成熟种子为外植体,研究了不同的种子处理方法和基本培养基、生长调节剂与壳聚糖的添加量对愈伤组织诱导的影响,以及愈伤组织的继代次数对植株分化的影响。结果表明:剥去外壳、划破种皮的结缕草种子在N6培养基中,2,4-D浓度为4 mg/L,添加4 g/L壳聚糖时,出愈率最高,达到100%,胚性愈伤的发生率也达到最高,为84.4%;用含0.1 mg/L 2,4-D的1/2MS分化培养基,经1次继代的浅黄色稍致密愈伤组织分化率最高,达到81.7%。

With mature Zoysia japonica weeds explanet,studied the influence of different treatment of weeds,basic medium and the addition of growth regulator and chitosan to the induction of callus and the effect of subculturing times on the plantlet regeneration.Compared on the frequency and the forms of callus,the best method to induce callus was in N6 medium,under dark condition and with the combination of 2,4-D（4 mg/L）and chitosan（4 g/L）.Under such condition,the induction frequency of callus was 100%,while the percentage of embryogenic callus was 84.4%.Regenerated plantlets with regeneration frequency 81.7% were obtained after transferring embryogenic callus to the 1/2MS medium with 2,4-D 0.1 mg/L.