葡萄叶片蛋白质双向电泳技术体系的建立

Study on the Conditions of 2-DE Analysis of Proteome in Grape Leaveas

现对葡萄叶片中总蛋白质的双向电泳技术体系进行了初步探讨,比较了不同等电聚集程序、不同pH胶条以及不同SDS-PAGE凝胶浓度的双向电泳效果。结果表明：葡萄叶片蛋白质使用等电聚焦程序B、pH范围为4~7的胶条和12%的SDS-PAGE凝胶可以得到较为理想的双向电泳分析结果;采用7 cm胶条最大可以分离出143个有效蛋白质点。

The method system of 2-dimensional electrophoresis（2-DE）of protein in grape leaves was studied,the results of 2-dimensional electrophoresis from different procedures of isoelectric focusing,different immobilized pH gradients strips and with different SDS-PAGE gel concentration were compared.The results showed that isoelectric focusing procedure B,immobilized pH gradients（IPG）strip with the linear range of pH 4~7 and 12% SDS-PAGE gel were the better choice,more than 143 protein spots were separated in one run.