野生乌桕的组培和再生

Tissue Culture and Regeneration of Wild Sapium sebiferum

为了建立乌桕的组培再生体系用于转基因育种,现对乌桕的芽分化和根系的再生进行了研究。以乌桕茎段为外植体,研究了不同消毒方法对外植体污染率,不同激素浓度(IBA、BA、NAA)和处理组合对芽分化和生根的影响。结果表明:升汞消毒10 min可降低外植体污染率至12.5%;细胞分裂素6-BA对乌桕芽的分化效果明显,各浓度处理中,以6-BA 1 mg/L处理的芽分化数最多;乌桕芽的分化还与IBA的浓度有关,最佳的芽分化配方是MS+6-BA 1.0 mg/L+IBA 0.1 mg/L,分化率达617%;最佳生根培养基是1/2MS+NAA 1.0 mg/L+IBA 0.1 mg/L,生根率达97.5%。

The bud differentiation and roots regeneration were researched with stem explants under different sterilized method,hormone treatments in order to set up regeneration system and genetic transformation of Sapium sebiferum.The results showed that the contaminated ratio decreased to 12.5% when explants were sterilized by HgCl2 and the bud differentiation rate was most in all treatment with 6-BA 1.0 mg/L.The IBA also affected the bud differentiation and the best treatment combination was MS＋6-BA 1.0 mg/L＋IBA 0.1 mg/L and the differentiation rate was 617%.The roots regeneration was 97.5% on culture medium of 1/2MS＋NAA 1.0 mg/L＋IBA 0.1 mg/L.