摘要
以山新杨(P.davidiana×P.bolleana)叶片为外植体,1/2MS为基本培养基,对适合山新杨叶片再生的生长调节剂6-BA和NAA进行研究。结果表明:适宜山新杨叶片分化培养基是1/2MS+NAA 0.05 mg/L+6-BA 0.5 mg/L;继代抽茎培养基是1/2MS+NAA 0.08 mg/L+6-BA 0.1 mg/L;最佳生根培养基是1/2MS+IBA 0.3 mg/L。
With Populus（Populus davidiana× P.bolleana）leaves as explants,1/2MS as the basic medium,this experiment＇s aim was to study the growth regulator 6-BA and NAA which fitted the leaf regeneration.The results showed that the most appropriate medium for Populus leaf differentiation was 1/2MS with NAA 0.05 mg/L and 6-BA 0.5mg/L,and the most appropriate subculture medium was 1/2MS with NAA 0.08 mg/L and 6-BA 0.1 mg/L,and the best rooting medium was 1/2MS with IBA 0.3 mg/L.
出处
《北方园艺》
CAS
北大核心
2010年第13期174-175,共2页
Northern Horticulture
关键词
山新杨
组织培养
分化
生根
populous
tissue culture
differentiation
root
