摘要
为了建立油葵基因转化体系,以pCB302为载体,进行了C58C1农杆菌介导的遗传转化。结果表明:3 mg/L的除草剂浓度为最适宜的选择压;叶片、叶柄和茎段3种外植体中,茎段的基因转化率最高;71、42、8 d的继代培养时间中,7 d的继代培养基因转化率最高。
In order to establish the system of genetic transformation on oil soybean by Agrobacterium medium transformation of Agrobacterium tumefaciens strains C58C1 with pCB302 vector,the concentration of herbicide,the selection of explants and pre-culture were researched.The results showed that more suitable concentration of herbicide was 3 mg/L,the more desirable explants were stems,the more desirable time of pre-culture was 7 days with highest transformation ratio.
出处
《北方园艺》
CAS
北大核心
2010年第13期176-178,共3页
Northern Horticulture
基金
天津市科委科技支撑资助项目(08ZCKFNC01200)
(07ZCKFNC01100)
教育部留学回国人员科研启动基金资助项目
关键词
油葵
基因转化
选择压
外植体
oil soybean
genetic transformation
explants
selection pressure
