血清TGF-β1和VEGF与脑梗死患者颈动脉粥样斑块易损性的相关性

Relations of transforming growth factor-β1 and vascular endothelial growth factor with carotid atherosclerotic plaques vulnerability in patients with cerebral infarction

目的探讨血管内皮细胞生长因子(VEGF)和转化生长因子-β1(TGF-β1)与脑梗死患者颈动脉粥样硬化斑块易损性的关系。方法收集96例急性期动脉粥样硬化性脑梗死患者和30名健康对照者。采取酶联免疫法检测血清VEGF和TGF-β1水平;采用彩色多普勒超声对脑梗死患者的颈动脉粥样硬化情况进行评估,并将脑梗死患者分为伴易损性颈动脉斑块组、伴稳定性颈动脉斑块组和无颈动脉粥样硬化组,分析血清VEGF和TGF-β1水平与脑梗死患者颈动脉粥样硬化易损斑块的相关性。结果脑梗死患者血清VEGF水平明显高于对照组,而TGF-β1水平明显低于对照组(P<0.01);伴易损性颈动脉斑块的脑梗死患者血清VEGF水平明显高于伴稳定性颈动脉斑块的脑梗死患者(P<0.01),而TGF-β1水平明显低于伴稳定性颈动脉斑块的脑梗死患者(P<0.01);VEGF水平与颈动脉斑块易损性呈正相关(r=0.493,P<0.01),而TGF-β1水平与颈动脉斑块易损性呈负相关(r=-0.524,P<0.01)。结论 VEGF和TGF-β1在脑梗死的发病和脑缺血损伤中可能起重要的作用,TGF-β1高表达和脑梗死患者颈动脉粥样硬化斑块稳定性之间存在密切联系,TGF-β1具有稳定颈动脉粥样硬化斑块的作用。

Objective To investigate the relations of vascular endothelial growth factor （VEGF） and transforming growth factor-β1 （TGF-β1） with carotid atherosclerotic plaque vulnerability in patients with cerebral infarction. Methods Ninety-six cases of acute atherosclerotic cerebral infarction and 30 healthy controls were f collected for study. The levels of VEGF and TGF-β1 in serum were measured by enzyme-linked immunosorbent assay. The degree of carotid atherosclerosis in 96 patients with acute atherosclerotic cerebral infarction was assessed by color Doppler ultrasound. According to the results, the patients were divided into three groups, the vulnerable carotid artery plaque group, the stable carotid artery plaque group and the no carotid artery atherosclerosis group. The relations of the levels of VEGF and TGF-β1 in serum with the vulnerability of carotid atherosclerotic plaques were analyzed in patients with cerebral infarction. Results The levels of VEGF in patients with cerebral infarction were significantly higher than those of the controls, while the levels of TGF-β1 were significantly lower （P〈0. 01）. The levels of VEGF in patients with unstable carotid atherosclerotic plaques were significantly higher than those with the stable carotid atherosclerotic plaques （P〈0. 01）, while the level of TGF-β1 in patients with unstable carotid atheroselerotic plaques was significantly lower than those with the stable carotid atherosclerotic plaques （P〈0. 01）. The levels of VEGF were positively related to the vulnerability of plaques （r=0. 493, P〈0.01）, while the level of TGF-β1 was negatively correlated with the vulnerability of plaques （r =- 0. 524, P 〈 0.01）. Conclusions VEGF and TGF-β1 might play important roles in the pathogenesis of cerebral infarction and cerebral ischemic injury. There was a close link between high expression of TGF-β1 and the stability of carotid atherosclerotic plaques. TGF-β1 could stabilize carotid atherosclerotic plaques.