池养三角帆蚌卵巢发育与卵子发生的组织学研究

Histological study on ovarian development and Oogenesis of Hyriopsis cumingii cultured in the pond

通过石蜡切片技术与苏木精-伊红染色的组织学方法,研究了池塘养殖条件下3龄三角帆蚌亲蚌卵巢发育及卵细胞发生过程的组织学特征。其亲蚌卵巢发育经历了5个时期:2月为增殖期,3月为生长期,4月开始进入成熟期,4月至11月为排放期,12月至1月为休止期。三角帆蚌卵子发生也经历了5个时期:卵原细胞期、生长初期、生长中期、生长后期和成熟卵母细胞期。三角帆蚌卵细胞发育不同步,分批成熟、分批排放。卵子发生初期,未观察到明显的卵柄结构;卵子发生中期,卵母细胞核中有一显著大核仁,但没有观察到小核仁。

By means of histological section and H. E. dyeing,the histological characters were studied in the process of the ovarian development and Oogenesis of Hyriopsis cumingii. The mussels were cultured in the pond which could be used to reproduce. The ovarian development of Hyriopsis cumingii could be divided into five stages： proliferating period in February,growing stage in March,maturing stage started in April,spawning stage during April to November,resting stage during December to January. The oogenesis of Hyriopsis cumingii could be also divided into five stages： oogonia,early vitellogenic oocytes,vitellogenic oocytes,late vitellogcnic oocytes,and mature oocytes. The oocytes matured and spawned in different time. In the early time of oogenesis,the yolk stalk was not observed obviously. A big nucleolus was observed in the middle time of oogenesis,but no small nucleolus was observed.