铜绿假单胞菌对两种消毒剂的抗性变化及其抗药基因检测

STUDY ON THE RESISTANT TENDENCY OF Pseudomonas aeruginosa TO TWO DISINFECTANTS AND DETECTION OF qacE-sul1 GENE

目的观察连续传代的铜绿假单胞菌临床株对邻苯二甲醛和碘伏的抗性变化及其与qacE-sul1基因的关系。方法采用肉汤稀释法、悬液定量杀菌试验和PCR方法,检测了两种消毒剂对连续传代的铜绿假单胞菌临床株最小抑菌浓度、最小杀菌浓度变化和抗药基因携带率。结果临床分离的铜绿假单胞菌经连续传代后对邻苯二甲醛、碘伏的抗性均逐渐增强,这些菌株qacE-sul1基因检测结果均为阴性。结论长期使用亚致死剂量的消毒剂可能导致铜绿假单胞菌的抗性产生或增强,其抗性变化与qacE-sul1基因无关。

Objective To study the resistance of Pseudomonas aeruginosa to ortho-phthaladehyde and iodophor by continuously passage and the relationship with qacE-sul1 gene.Methods Broth dilution method and suspension quantitative germicidal tests were used to observe the change of minimal inhibitory concentration and minimal bactericidal concentration of continuously passage Pseudomanas aeruginosa against two disinfectants,and polymerase chain reaction was used to detect the positive rate of qacE-sul1 gene.Results The resistance of clinical isolated Pseudomonas aeruginosa was enhanced gradually to orhto-phthaladehyde and iodophor by continuously passage.The experimental strains were all negative of qacE-sul1 gene.Conclusions The resistance may be caused by exposed to the sublethal disinfectant for a long time.No relationship was observed between the resistance to disinfectants and qacE-sul1 gene.