氟对不同世代大鼠大脑皮质细胞凋亡的影响

Apoptosis in Brain Cells of Different Generations in Rats Exposed to Fluoride

为研究氟中毒能否引起世代蓄积而对不同世代动物大脑造成损伤,试验采用离乳Wistar大鼠16只,随机分为2组,对照组饲喂大鼠标准饲料,饮自来水;高氟组饲喂大鼠正常饲料,饮含氟化钠(NaF)100 mg/L的去离子水。饲喂3个月,生产子二代和子三代大鼠,仔鼠饲喂采用与其母鼠相同的处理。取0、10、20、30、60、90日龄的不同世代大鼠,分别宰杀,取大脑,制成单细胞悬液,采用流式细胞仪检查其细胞凋亡情况。结果显示,与对照组相比,不同日龄高氟组二代、三代大鼠大脑皮质细胞凋亡率升高。与二代大鼠相比,不同日龄氟中毒三代大鼠大脑皮质细胞凋亡程度增高,10、20、60、90日龄时差异显著(P<0.05)。提示,氟中毒可以引起世代蓄积,接触氟环境时间越长,其动物子代大脑损伤越严重。

To assess brain cell apoptosis induced by fluoride in their offspring,16 one-month old Wistar albino rats were divided randomly into two equal groups,each with six females and two males.The first group of rats served as the untreated controls;the second group received high fluoride（HiF） in their drinking water（100 mg/L NaF）;After the animal model was established,the rats were allowed to breed,and 16 offspring rats in each group were randomly selected for the experiments.The treatment for these second and third generation rats was the same as for their parents.At 0,10,30,60 and 90 days after birth,these offspring rats were anesthetized and their brain cells prepared for flow cytometry.In comparison with the controls,the percent of brain cell apoptosis in the offspring rats in fluoride groups was obviously higher,especially in third generation rats.In comparison with the second generation,brain cells apoptosis in different aged third generation rat was increased,especially at 10,20,60 and 90 days（P0.05）.These results indicate that fluoride can accumulate in different generation,and the longer the period sustained,the severer damage of the brain in offspring animal occurred.