摘要
本研究利用内蒙古白绒山羊的7个父系半同胞家系共794个个体,用X染色体上的7个微卫星标记进行了系谱确认,构建了绒山羊X染色体遗传连锁图。结果表明,7个标记的等位基因数变化范围为9～14,杂合度在0.585～0.918之间,平均杂合度为0.726;多态信息含量在0.759～0.897之间,平均多态信息含量为0.834。构建的内蒙古白绒山羊X染色体遗传连锁图总长度139.4 cM,与英国罗斯林研究所公布的SM4.7绵羊连锁图标记顺序一致,可用于下一步的QTL定位研究。
The 7 microsatellite markers on chromosomes X were used to construct a linkage map of Cashmere goats with a 794 members half-sib pedigrees after paternity testing.The results showed that the number of alleles for 7 markers was 9 to 14,the heterozygosity was 0.585 to 0.918,the average heterozygosity was 0.726,and polymorphic information content（PIC） was 0.759 to 0.897,the average polymorphic information content was 0.834.Length of the linkage map of chromosome X we built was 139.4 cM,and the sequence of loci was coincident with the SM4.7,and could be used in quantitative trait loci（QTL） searching in Cashmere goats.
出处
《中国畜牧兽医》
CAS
北大核心
2010年第7期109-112,共4页
China Animal Husbandry & Veterinary Medicine
基金
国家科技支撑计划(2007BAD56B3)
关键词
绒山羊
微卫星标记
X染色体
连锁图
Cashmere goat
microsatellite markers
chromosomes X
linkage map
