5个猪种DECR1基因外显子2单链构象多态性的群体遗传学分析

Population Genetic Analysis of Polymorphisms on Exon 2 of DECR1 Gene in 5 Pig Breeds

试验利用PCR-SSCP技术检测了山西马身猪、山西白猪、长白猪、大白猪和杜洛克猪5个品种共490头猪的DE-CR1基因外显子2的多态性。结果显示,DECR1基因外显子2位点存在多态性,表现出AA、BB和AB 3种基因型。群体遗传学分析结果表明,5个猪种都是等位基因B为优势基因;Hardy-Weinberg平衡检验结果显示,山西白猪和杜洛克猪处于平衡状态,山西马身猪、长白猪和大白猪处于非平衡状态;各品种猪的多态信息含量均为中度多态。

The polymorphisms of the 2,4-dienoyl CoA redutase 1（DECR1） gene in exon 2 site was detected using PCR-SSCP in 490 pigs of 5 pig breeds including Shanxi Mashen,Shanxi White,Landrace,Yorkshire and Duroc.The results showed that there was a polymorphism in the exon 2 site and three genotypes（AA,BB and AB） were found.Analysis of population genetics suggested that the frequency of B allele was dominant among the studied breeds.The results of Hardy-Weinberg equilibrium test indicated that Shanxi White and Duroc reached the equilibrium.All of the breeds had intermediate polymorphism information contents.