核酸探针检测山东省鸡群中马立克病毒、传染性贫血病毒、网状内皮增生病病毒和呼肠孤病毒的流行状况

Epidemic Investigation of Infection of MDV,CAV,REV and REOV from Broiler in Shandong by Dot Blotting

为对山东家禽业免疫抑制病的流行情况进行调查,从烟台、滨州、临沂、泰安、聊城、青岛大型养殖场共随机收集600只病死鸡的肝脏和脾脏病例组织样品,用斑点杂交的方法检测样品中鸡马立克病毒(Marek's disease virus,MDV)、传染性贫血病毒(chicken anemia virus,CAV)、网状内皮增生病病毒(reticuloendotheliosis virus,REV)和呼肠孤病毒(reovirus,REOV)的感染情况。结果发现,MDV、CAV、REV、REOV的感染率分别为5.17%、8.17%、1.17%、2.5%,检出率较以前的研究报道有降低趋势,有多种双重感染和三重感染,表明山东家禽业在免疫抑制病方面总体控制较好,但不同的养殖场发病情况差异极显著,管理水平参差不齐,个别地区发病情况严重,检出率超过其他地区的总和。

The liver and spleen samples of 600 unhealthy chickens were collected randomly to investigate infection of MDV,CAV,REV and REOV in Shandong province using Dot blotting.The clinic tissues came from six different districts including Yantai,Binzhou,Linyi,Tai＇an,Liaocheng and Qingdao of Shandong.The results suggested that the checkout ratios of the four virus are 5.17%（MDV）,8.17%（CAV）,1.17%（REV） and 2.5%（REOV） respectively which were lower than the former research;certain degree duale infection and triple infection were found out.The result indicated that the immunodepressive virus are well controlled except individual flock in which the rate of infection was higher than the sum of other flocks;there was a significant discrepancy of the immunodepressive illness in different flocks where the management level were uneven.