自制貉源抗血清治疗犬瘟热病的研究

The Study of Self-made Anti-serum Being Used to Cure Canine Distemper that was Made from Raccoon

应用含有104.5TCID50/0.1 mL犬瘟热弱毒培养物4 mL免疫特种经济动物乌苏里貉,10 d后采集血液分离血清,病毒用200TCID50与等量连续倍比稀释的血清和经饱和硫酸铵粗提的免疫球蛋白做中和试验鉴定抗体的效价,并对上述2种抗体效价进行比较。结果表明,2917倍稀释全血清、2344倍稀释饱和硫酸铵粗提的免疫球蛋白能保护50%Vero细胞不出现CPE。临床应用全血清治疗24只患犬瘟热病的白貉,22只成活。由此说明可以应用狐貉等特种动物制备的抗血清治疗犬瘟热病。

In this study,4 mL canine distemper virus being 104.5 TCID50/0.1 mL,was used to immunize wusuli-raccoon dog which was strain economic animal.After 10 days,animal blood was collected,while blood serum was disassociated.Neutralization test was made to evaluate the potency of antibody,during this test,virus was 200TCID50,blood serum whose volume was equal with virus was diluted by times after times,while immunoglobulin was extracted crudely by saturated ammonium sulfate.The results clearly suggested that under the project that the blood serum was diluted for 2917 times,the immunoglobulin which was extracted crudely by saturated（NH3）2SO4 was diluted for 2344 times,there was 50% Vero cytoprotection without CPE.24 white raccoon dogs with this disease were therapied by the whole serum above,and 22 ones was survival,therefore,we demonstrated that antiserum,made from strain animal such as fox,could be used tocure canine distemper virus.