人工感染绦虫条件下猪血清中免疫球蛋白E的分离提取与鉴定

The Extraction and Identification of Porcine Serum IgE in Condition of Artificial Infection

注射疫苗可以引起猪发生过敏反应,尤其品种猪发病率较高,治疗不及时极易导致动物死亡。过敏反应主要由免疫球蛋白E(immunoglobulin E,IgE)介导发生,分离纯化到猪体内的IgE是研究猪过敏反应ELISA检测方法的前提和基础。IgE在正常人血清中浓度极低,正常猪血清中IgE含量还未见有确定报道。本试验研究的目的是通过人工感染方法对感染绦虫试验猪血清样品中的IgE进行提取,并且通过SDS-PAGE电泳对血清中提取的IgE进行鉴定,从而建立猪血清IgE的最佳提取方法。

Porcine allergic action can be caused by injecting vaccines,especially some species.Allergic action can cause animal death without curing in time.Allergic action is mainly mediated by IgE（immunoglobulin E）.Separation and purification of porcine serum IgE is the precondition and foundation of detecting porcine allergic action by ELISA.IgE＇s content is minimum in health adult,porcine serum IgE level hasn＇t accurate report.The purpose of this experiment was that through extract IgE from blood serum of artificial infected cephalocyst and identify serum IgE by SDS-PAGE,thereby create the best method to extract IgE from porcine serum.