4-氨基联苯与人体血红蛋白体外加合反应及加合物测定的研究

In vitro adduct formation of hemoglobin with 4 amino biophenyl

研究了人体血红蛋白（Ｈｂ）与４氨基联苯（４ＡＢＰ）加合物的测定方法．考察加合物的分离条件及定量测定加合物的精确度．研究人体血红蛋白的体外加合产物的生成量与反应时间的关系．体外实验结果发现，在３７℃恒温条件下，１２ｈ之内，４ＡＢＰ与Ｈｂ加合物的生成速率为１２６ｐｇ／（ｍｇ·ｈ）．

Reliable determination method for 4 aminobiphenyl adduct with human hemoglobin in vitro was developed. The separating conditions of the adduct from low molecule weight ingredients and the precision of quantity inspection for the adduct were examined. Adduct reaction of 4 aminobinbenyl to hemoglobin occurred directly according to the result of the adduct analysis. It was confirmed that the rate of 4 ABP binding to Hb in vitro is 126 pg/(mg(Hb)·h) within 12 hours at 37℃.