胃癌细胞产生的一种新型内皮细胞生长因子的分离

Isolation of a Novel Endothelial Cell Growth Factor Derived from Gastric Cancer Cell

人胃癌细胞株 NUGC 2的培养上清液对内皮细胞及纤维细胞有生长刺激作用.收集 NUGC 2的培养上清液,然后经硫酸铵盐析。等电聚焦(IEF)、Mono Q 阴离子交换层析柱、凝胶过滤层析柱(TSK Gel G3000 SW)等方法分离蛋白,得到一种只对内皮细胞有生长刺激作用,对纤维细胞没有生长刺激作用化学不均一的内皮细胞生长因子,其等电点(PI)为2.07～2.90,分子量在45 000～66 000范围,盐洗脱浓度为0.1～0.25M NaCl.它不同于酸性纤维细胞生长因子,也不同于碱性纤维细胞生长因子.

The culture supernatant was found to stimulate endothelial cell and fibroblast proliferation.It was deemed possible that the supernatant contained endothelial cell growth factor the NUGC 2 culture supernatant was collected and purified by salt fractional,isoelectric focusing electrophoresis (IEF),ion exchange chromatography (Mono Q),and gel filteration (TSK Gel G 3000 SW).In the end a chemical heterogeneous growth factor was obtained.The factor only stimulated the prolifera- tion of endothelial cell but not fibroblase cell,its molecule weight was about 45 000-66 000,PI 2.07-2.90,salt eluted concentration 0.1-0.25 M NaCl.