实验性缺血性脑卒中各脑区脑啡肽时相变化

Met-enkephalin Levels following Experimental Cerebral Ischemia in Gerbil Brain

本实验用特异性兔抗甲硫氨酸脑啡肽(met-enkephalin.MEK)对正常和受到30分钟、60分钟及120分钟双侧颈总动脉结扎造成缺血模型的蒙古沙土鼠各脑区进行放射免疫测定,观察各脑区含量变化.结果显示,脑缺血30分钟时,下丘脑含量显著升高(P<0.05),以后逐渐下降至对照组水平,而海马、皮层、纹状体的含量显著下降(P<0.01;P<0.05),且持续至120分钟.结果提示 MEK 有可能通过直接或间接途径参与缺血的病理过程.

Met-enkephalin (MEK) in different regions of Mongolian gerbil brain was measured by radioimmunoassay at 30,60,120 minutes after bilateral common carotid artery occlusin.Following 30 minutes of ischemia,a signifcant increase of MEK concentration was observed in the hypothalamus,returning to the control level at 120 minutes.In contrast,a signifcant decrease was detected in the hippocampus, cerebral cortex,striatum and the lower values persisted for 120 minutes.Our results support the notion that MEK may be involved directly or indirectly in the pathophysiology of cerebral ischemia.