ELISA法检测HBsAg出现假阳性的原因探讨

Enzyme-linked Immunosorbent Assay of Hepatitis B Surface Antigen Occurred Because of False Positive

目的探讨采用ELISA法检测HBsAg出现假阳性的原因。方法采用ELISA法检测的不同年龄段人群乙肝表面抗原阳性率,并与采用胶体金标法检测作比较。结果采用ELISA法检测的乙肝表面抗原阳性率敏感性为92.3%(48/52),明显高于采用胶体金标法检测的乙肝表面抗原阳性率,敏感性为72.7%(48/66),特异性为96.0%(430/448),略低于采用胶体金标法检测的特异性99.1%(430/434)。采用ELISA法检测的乙肝表面抗原的假阳性率为4.02%(18/448),假阴性率为7.69%(4/52),χ2=8.34,P<0.05。结论 ELISA法检测乙肝HBsAg灵敏度高、特异性强,易造成假阳性。

Objective To investigate the detection of HBsAg by ELISA,false positive reason. Methods ELISA assay of the different age groups the rate of hepatitis B surface antigen positive, and with the use of colloidal gold standard assay for comparison. Results ELISA detection of hepatitis B surface antigen positive rate sensitivity of 92.3%（48/52）,significantly higher than the standard method using colloidal gold detection of hepatitis B surface antigen positive rate, sensitivity of 72.7%（48/66）,specificity was 96.0%（430/448）, slightly lower than the gold standard method using the specificity of detection. By ELISA assay of hepatitis B surface antigen false positive rate was 4.02%（18/448）and the false negative rate was 7.69%（4/52）, χ^2=8.34,P〈0.05. Conclusion ELISA detection of hepatitis B HBsAg high sensitivity and specificity, could easily lead to false positive.