滇西南地区微小按蚊(Anopheles minimus)实验室驯化种群的建立

Anobservation on Domestication to Anopheles minimus from south—west areas of yunnan province in laboratory

为开展对云南微小按蚊遗传多态性与传疟作用的研究需建立实验室品系。将羽化后4d成蚊进行自然交配试验,结果550只雌蚊产卵1192枚,平均2.17枚;孵化、化蛹、羽化率分别为63.51％、62.48％、87.53％,获第1代自然交配繁殖成蚊414只。然后采用人工交配与自然交配繁殖同龄成蚊混合驯化观察3代,产卵从最初的平均2.17枚提高到9.22枚;孵化率也从63.51％上升到92.67％。实验表明此法是一种简便、快速、有效的驯化方法。

In order to carry out the study about the inheritant polypeptide and transmission malaria,it was necessary to establish the strain of labiratory. This study conducted the test of natural mating to this mosquito after emergence fourth day. The results are lay egg 1992 in 550 female mosquitods, average 2. 17 per female mosquito,the rate of hatch,pupa and emergence is 63. 51 % ,62. 48% and 87. 53% ,respectively. It was gained 414 in mutural mating. And Then it was donesticated for Three generations mixed with the artificial and nutural muting. The rate of lay egg has increused from 2. 17 initully to 9. 22 and the rate of hatch from 63. 51% to 92. 67%. This tudy shown the way is a simple,faster and effective method in domestication.