转化生长因子β基因在BXSB狼疮小鼠肾脏中的表达

Expression of Transforming Growth Factor β Genes in Kidneys of BXSB Lupus Mice.

目的研究转化生长因子（ＴＧＦβ）与狼疮性肾炎进展的关系。方法采用同位素标记ｃＤＮＡ探针Ｎｏｒｔｈｅｒｎ印迹杂交方法，检测了自发狼疮性肾炎的ＢＸＳＢ小鼠肾脏组织中ＴＧＦβｍＲＮＡ水平。结果发现与狼疮性肾炎发病较轻的６月龄雌性ＢＸＳＢ小鼠相比较，狼疮性肾炎发病较重的６月龄雄性ＢＸＳＢ小鼠肾脏表达ＴＧＦβｍＲＮＡ的量显著增加（Ｐ＜０．０１），是对照雌鼠表达量的６倍。结论提示ＴＧＦβ可能在狼疮性肾炎肾小球细胞外基质的病理性积聚和肾小球硬化的形成过程中起重要作用。

Objective In order to study the relationship between transforming growth factor(TGF)β and lupus nephritis progression.Method Levels of mRNA for TGF β in the kidneys of BXSB mice with lupus nephritis were detected by Northern blot hybridization with 32 P isotope labeled probe.Results 6 month old BXSB male mice with active lupus nephritis expressed signififcantly elevated concentrations of mRNA when compared with that of 6 month old female BXSB mice as controls( P <0.01).Levels of mRNA for TGF β showed 6 fold increase compared with that of control mice.Conclusion Increased expression of TGF β mRNA may play a important role in the glomerular accumulation of pathological extracellular martix in lupus nephritis and contribute to glomerulosclerosis in these SLE model.