转化生长因子β1对口腔鳞状细胞癌脑转移细胞系Tb细胞生长抑制作用的研究

Inhibitory effect of transforming growth factor-β1 on oral squamous cell carcinoma brain metastasis Tb cell line

目的 探讨转化生长因子（transforming growth factor,TGF）β1对口腔鳞状细胞癌脑转移细胞系Tb细胞的生长抑制作用及可能的机制.方法 采用细胞计数法检测TGF-β1对Tb细胞的生长抑制作用,流式细胞仪检测细胞周期的变化,微阵列分析法（superarray）筛查smad蛋白介导的TGF-B（TGF-β-Smads）信号通路中基因表达水平的变化,反转录聚合酶链反应（RT-PCR）验证差异表达基因.结果 TGF-β1对Tb细胞有明显的生长抑制作用（P〈0.05）.流式细胞仪检测显示,TGF-β1能够使Tb细胞周期阻滞于G1期（P〈0.05）.微阵列分析法筛查结果显示,TGF-β1作用后,活化素受体样激酶1（activin receptor-like kinase-1,ACVRL-1）、抗苗勒激素（anti-mullerian hirmine,AMH）、细胞周期蛋白依赖性激酶抑制因子2B（cyclim-dependent kinase inhibitor-2B,CDKN-2B）、TGF-相互作用因子（indnced factor,TGIF）基因表达增加,而畸形肿瘤衍生生长因子1（teratocarcinomaderived growth factor-1,TDGF-1）基因表达降低.RT-PCR验证结果表明,ACVR-1（0.67±0.08）、CDKN-2B基因表达（2.16±0.95）与微阵列分析法结果一致,差异有统计学意义（P〈0.05）,AMH（0.38±0.07）、TDGF-1（0.44±0.06）及TGIF（0.52±0.10）基因表达水平无统计学意义（P〉0.05）.结论 TGF±β1对口腔鳞状细胞癌脑转移细胞系Tb细胞有明显的生长抑制作用,其机制可能与细胞周期调控及调节TGF-β1-Smads信号通路中ACVRL-1、CDKN-2B基因表达有关.

Objective To investigate the inhibitory effect of transforming growth factor （TGF） -β1 on oral squamous cell carcinoma （OSCC） Tb cell line.Methods Cell counting method was used to examine the inhibitory effect of TGF-β1 on Tb cell and flow cytometry（FCM） assay performed to measure the changes of cell cycle.Superarray was used to screen the changing expression of genes in TGF-β1/Smads signaling pathway.RT-PCR method was used to detect the results of Superarray.Results TGF-β1 showed significant inhibiting effect on OSCC Tb cell line.TGF-β1 blocked the cell cycle at G1 phase.The expression level of activin receptor-like kinase -1 （ACVRL-1）,anti-mullerian hirmine （AMH）,cyclim-dependent kinase inhibitor-2B （CDKN-2B） and transforming growth factor-beta-indnced factor（TGIF） was higer in the cells treated with TGF-β1 than in control,while TDGF-1 expression was down-regulated.ACVRL-1 and CDKN-2B gene expression was consistent with the results of Superarray.Conclusions TGF-β1 can inhibit the growth of OSCC Th cell line.The mechanism may be related to the regulation of cell cycle and the expression of ACVRL-I and CDKN-2B in TGF-β1-Smads signaling pathway.