鲍曼不动杆菌中16S rRNA甲基化酶基因的检测

Detection of 16S rRNA methylase gene in Acinetobacter baumannii

目的了解耐氨基糖苷类药物鲍曼不动杆菌中16S rRNA甲基化酶基因的流行情况。方法收集2008年1月—2009年4月临床分离对阿米卡星和庆大霉素耐药的鲍曼不动杆菌70株,PCR(聚合酶链反应)方法扩增5种甲基化酶基因(armA、rmtA、rmtB、rmtC和rmtD),PCR产物进行电泳分析和测序。统计菌株对亚胺培南等12种抗生素的耐药率。结果 armA基因的检出率为68.6%,未检出rmtB、rmtA、rmtC和rmtD基因。所有菌株对除亚胺培南外其它抗生素的耐药率均大于80%。结论 16S rRNA甲基化酶(armA亚型)基因广泛存在于这些耐药菌株中。这些对阿米卡星和庆大霉素耐药的菌株,对其它抗生素的耐药也十分严重。

Objective To investigate the prevalence of 16S rRNA methylase and aac（6′）-Ib-cr gene in aminoglycoside-resistant acinetobacter baumannii.Methods A total of 70 amikacin-resistant and gentamicin-resistant acinetobacter baumannii were collected from January 2008 to April 2009.ArmA,rmtA,rmtB,rmtC and rmtD gene were detected by PCR-based asays.Some positive PCR products were sequenced.Statistics resistant rates of clinical isolates to 12 antibiotics.Results The prevalence rates of armA gene were 68.6%,no rmtB,rmtA,rmtC and rmtD gene was detected in 70 acinetobacter baumannii.Sistant rates of 70 strains to other antibiotics were more than 80%,expect imipenem.Conclusion 16S rRNA methylase armA gene existed widely in clinical isolates of amikacin-resistant and gentamicin-resistant acinetobacter baumannii.These acinetobacter baumannii also confer high-level resistance to other antibiotics.