摘要
本研究运用已经获得的1条490bp的普通烟草钾离子(K^+)通道基因的中间片段来设计特异性引物,应用RACE方法获得其3'和5'末端cDNA序列。通过三段cDNA序列拼接结合全长克隆测序验证,获得一个未报道的普通烟草K^+通道基因,并将其命名为NKT5(NCBI登录号为HM010922)。NKT5的cDNA全长为2365bp,其中5'端非编码区190bp、3'端非编码区249bp、编码区1926bp,编码641AA。对该基因进行了序列分析及功能预测,为进一步阐明其在烟草吸钾及抗逆过程中的功能及分子机制奠定了基础。
Based on a 490 bp middle segment of K ion channel gene,a pair of specific primers was designed to acquire 5＇ and 3＇-ends of cDNA by RACE strategy.An unreported K ion channel gene in tobacco（Nicotiana tabacum） named as NKT5 was obtained by splicing three cDNA sequences and sequencing full-length cloning.The full-length cDNA of NKT5 is 2 365 bp long with 190 bp 5＇-untranslated region（UTR）,249 bp 3＇-UTR and 1 926 bp coding sequence.The deduced amino acid is 641 AA long.The gene sequence analysis and function prediction were carried out in order to clarify molecular mechanisms of potassium absorption and stress-resistance in the tobacco.
出处
《中国烟草科学》
CSCD
北大核心
2010年第4期8-13,共6页
Chinese Tobacco Science
