摘要
研究了不同浓度的茉莉酸甲酯(MeJA)对烟草α-淀粉酶抑制剂诱导的时间及浓度效应。在斜纹夜蛾取食不同浓度的MeJA诱抗处理的烟草叶片1～3d后,对中肠α-淀粉酶活性进行了检测。结果显示,0.01,0.1,1.0,4.0mmol/L和10.0mmol/L5种浓度的MeJA处理烟草后,烟叶α-淀粉酶抑制剂含量明显增加,而4.0mmo1/L MeJA诱抗处理48h时诱导烟草产生的α-淀粉酶抑制剂活性最强。离体检测结果表明,斜纹夜蛾取食MeJA处理的烟草叶片后,中肠α-淀粉酶活性降低。
The time and concentration inducement effects of methyl jasmonate（MeJA） on а-amylase inhibitor in tobacco leaves were studied,and the activities of Spodoptera litura（F.） midgut α-amylase was measured,which feed on the tobacco leaves after 1-3d MeJA spraying.The results showed that,after treated with five concentrations of MeJA（0.01,0.1,1.0,4.0,10.0 mmol/L）,the content of α-amylase inhibitor activity in leaf increased significantly.The 4 mmol/L MeJA induced the strongest response of а-amylase inhibitor activity in tobacco leaves after 48 h treatment.In vitro assay demonstrated that the Spodoptera litura（F.） midgut а-amylase activity decreased after feed on the treated tobacco leaves.
出处
《中国烟草科学》
CSCD
北大核心
2010年第4期20-24,共5页
Chinese Tobacco Science
基金
国家烟草专卖局项目"‘无公害’烟叶生产技术研究与应用"(110200202002)
