黑松抗氧化酶系统对海水胁迫的响应

Response of Antioxidative Enzymes of Pinus thunbergii Parl to Seawater Stress

以1 a生黑松幼苗为试材,研究了不同浓度海水胁迫下的黑松酶促抗氧化保护系统(超氧化物歧化酶SOD、过氧化物酶POD)。结果表明:在海水浓度组,SOD活性比对照组高出-32.60%～9.26%,POD活性比对照组高出-524%～209%;在1/2海水浓度下,SOD活性比对照组高出28.72%～39.23%,POD活性比对照组高出-92.2%～88.23%;在1/3海水浓度下,SOD活性比对照组高出35.04%～48.33%,POD活性比对照组高出-84.77%～27.50%;在1/4海水浓度下,SOD活性比对照组高出9.09%～91.67%,POD活性比对照组高出-27.15%～299%;在1/5海水浓度下,SOD活性比对照组高出8.71%～67.88%,POD活性比对照组高出-21.14%～185%。黑松幼苗在一定海水浓度范围和胁迫时间内,能通过自身诱导抗氧化酶系统提高适应能力以适应逆境。

Choose annual Pinus thunbergii Parl seedlings as the experimental materials to study Pinus thunbergii enzymatic antioxidant protection system（superoxide dismutase SOD,peroxidase POD）under different concentrations of seawater stress.The results showed that in the seawater concentration group,SOD activity higher than the control group by-32.60%～9.26%,POD activity higher than the control group by-524%～209%;in 1/2 seawater concentration,SOD activity higher than the the control group by 28.72%～39.23%,POD activity higher than the control group by-92.2%～88.23%;in 1/3 seawater concentration,SOD activity higher than the control group by 35.04%～48.33%,POD activity higher than the control group by 84.77%～27.50%;in 1/4 seawater concentration,SOD activity higher than the control group by 9.09%～91.67%,POD activity higher than the control group by-27.15%～299%;in 1/5 seawater concentration,SOD activity higher than the control group by 8.71%～67.88%,POD activity higher than the control group by-21.14%～185%.Pinus thunbergii Parl seedlings in a certain salt concentration range and stress time can using self-induced antioxidant enzyme system to increase adaptive capacity to adapt to adversity.