甜樱桃砧木高倍快繁技术研究

Study on Tissue Culture and Rapid High Rate Propagation of Sweet Cherry Rootstock

以ZY-1 1 a生休眠芽为试材,MS为基本培养基,通过调节6-BA和NAA的浓度,对甜樱桃矮化砧木ZY-1进行了高倍快繁技术研究。结果表明:MS+6-BA1.5 mg/L+NAA0 mg/L为多芽分化较适合培养基,它对提高芽分化率,增加苗数最有利;在继代培养中,以培养基MS+6-BA1 mg/L+NAA0.1 mg/L的增殖率最高,达37倍左右。

In this experiment,short-stemmed rootstock of sweet cherry induction in vitro based on ZY-1 were studied.Effective multiplication method was set up.The results showed that by adjusting the concentration of 6-BA and NAA in MS medium,MS＋6-BA 1.5 mg/L＋NAA 0 mg/L was effective for the shoot multiplication as a shoot inducing medium.In seedling subculture,MS＋6-BA 1 mg/L＋NAA 0.1 mg/L was available for the multiplication,and the rate was the highest to be 37.