摘要
Intermolelular communication between a receptor incorporated into liposome and lactate dehydrogenase(LDH) adsorbed on the membrane was established on artificial membranes,mediated by Cu2+.The receptor,having a moiety of pseudo-crown ether binding K+ and two histidine residues binding Cu2+,is a gemini compound with heteroditopic ion recognition site of Cu2+ as an inhibitor of the enzyme and K+ as a stimulating signal.The relative activity of the enzyme was 40.9% due to the inhibition of Cu2+ in the absence of K +,up to 85.9% in the presence of K +.In the communication system,the K+ signal was transduced to LDH by the receptor,leading to enhancement of LDH activity.
Intermolelular communication between a receptor incorporated into liposome and lactate dehydrogenase（LDH） adsorbed on the membrane was established on artificial membranes,mediated by Cu2＋.The receptor,having a moiety of pseudo-crown ether binding K＋ and two histidine residues binding Cu2＋,is a gemini compound with heteroditopic ion recognition site of Cu2＋ as an inhibitor of the enzyme and K＋ as a stimulating signal.The relative activity of the enzyme was 40.9% due to the inhibition of Cu2＋ in the absence of K ＋,up to 85.9% in the presence of K ＋.In the communication system,the K＋ signal was transduced to LDH by the receptor,leading to enhancement of LDH activity.
出处
《化学研究与应用》
CAS
CSCD
北大核心
2010年第7期930-933,共4页
Chemical Research and Application
基金
国家自然科学基金(20872013)资助项目
辽宁省教育厅基金(2009A154)资助项目
大连民族学院引进人才启动基金(20096102)资助项目
关键词
脂质体
分子间通讯
乳酸脱氢酶
纳米器件
liposome
intermolecular communication
lactate dehydrogenase
nanodevice
