钩藤的HPLC指纹图谱研究

HPLC Fingerprint of Ramulus Uncariae Cum Uncis

目的建立钩藤药材的HPLC色谱指纹图谱。方法以钩藤碱为参照物,采用HPLC法,色谱条件:Kro-mosil C18(250 mn×4.6 mm,5μm);流动相为甲醇-10 mmol磷酸二氢铵溶液,梯度洗脱进行色谱分离;检测波长:254nm;流速:1.0 ml.min-1;柱温:室温。结果 12批不同产地的钩藤药材中,除4批外,其余8批与系统生成的对照指纹图谱的相似度均在0.97以上;归纳出钩藤药材15个共有峰;通过与对照品的保留时间比较,11、12号峰分别为钩藤碱和异钩藤碱;不同产地钩藤药材中主要化学成分组成相似,但相对比例有明显的差异。结论所建立的方法简单可行,能有效控制钩藤药材的质量。

Objective To study the fingerprint of Ramulus Uncariae Cum Uncis by HPLC. Methods Rhynchophylline was used as the reference compound. The chromatographic condition of HPLC was as follows： Kromosil column C18 （250 mn×4.6mm,5μm）at room temperature, the mobile phase being methanol with 10 mmol·L^-l ammonium dihydrogen phosphate, gradient elution in chromatographic separation, the detective wavelength being 254 nm, and flow rate being 1.0 ml·min^-1. Results Except for four batches of 12 batches of Ramulus Uncariae Cure Uncis, thefingerprint similarity value of the other eight batches was above 0.97 as compared with system generation spectrum, and 15 common characteristic peaks were presented. The llth peak was identified as Rhynchophylline and the 12 th peak as isorhynchophylline compared with the retention time of standard sample. The results showed that the main chemical constituents of Ramulus Uncariae cun Uncis were similar among herbs from different habitats, but their rela- tive ratios are significantly different. Conclusion The method is simple, accurate and reproducibility, and can be used for the quality control of Ramulus Uncariae Cure Uncis.