3'-大豆苷元磺酸钠对前列腺癌细胞的影响

3'-daidzein sulfonate sodium on prostate cancer cells and the impact

目的:通过观察3'-大豆苷元磺酸钠对前列腺癌细胞增殖和相关基因表达的影响。方法:将前列腺癌细胞在培养基(含10%小牛血清)中采用开放式单层贴壁培养,采用MTT法对前列腺癌细胞的增殖情况进行分析,对3'-大豆苷元磺酸钠作用后的雄激素非依赖型前列腺癌细胞的存活率、细胞毒作用进行了研究。结果:与对照组相比,3'-大豆苷元磺酸钠对前列腺癌细胞抑制效应具有剂量依赖性,具有诱导前列腺癌细胞凋亡和引起坏死效应,同时诱导前列腺癌细胞的(PTEN基因)表达。结论:3'-大豆苷元磺酸钠具有明显抑制前列腺癌细胞增殖效应。

Objective ：To observe 3′-daidzein sulfonate sodium（DSS） of prostate cancer cell proliferation. Methods： Prostate cancer cell in the medium （ including 10% ealfserum） used in opened single-layer adherent euhure, adopted by MTT on the proliferation of prostate cancer cell an analysis of DSS role of the androgen - dependent prostate cancer cell survival, a eytotoxieity research. Results：Compared the solvent and the control group, DSS on prostate cancer cell inhibited in a dose-dependent effect, can induce apeptosis and cause necrosis effect, induced by prostate cancer cells and PTEN gene expression. Conclusion： DSS inhibits prostate cancer cell proliferation effect of prostate cancer cell.