胡蔓藤中非生物碱类成分的分离与鉴定(Ⅲ)

Isolation and identification of the non-alkaloid constituents from whole plant of Gelsemium elegans Benth.(Ⅲ)

目的研究胡蔓藤(Gelsemium elegans Benth.)中的化学成分。方法采用硅胶柱色谱和ODS柱色谱进行分离,Sephadex LH-20及制备液相进行纯化,根据理化性质和光谱分析进行结构鉴定。结果分离鉴定了8个化合物,分别为(+)-8-hydroxypinoresinol(1)、cleomiscosin C(2)、cleomiscosinA(3)、3,4-二羟基苯甲醛(3,4-dihydroxyphenyl aldehyde,4)、咖啡酸(caffeic acid,5)、1-O-咖啡酰基奎宁酸(1-O-caffeoylquinic acid,6)、4-O-咖啡酰基奎宁酸(4-O-caffeoylquinic acid,7)、1-O-咖啡酰基奎宁酸甲酯(1-O-caffeoylquinic acid methyl ester,8)。结论化合物1-8为首次从该属植物中分离得到。

Objective To study chemical constituents of whole plant of Gelsemium elegans Benth.Methods The constituents were firstly isolated by column chromatography with silica gel and flash ODS,then purified by Sephadex LH-20 and pHPLC.Structures of these compounds are identified on the basis of physico-chemical and spectral data.Results Eight compounds were isolated and identified as（＋）-8-hydroxypinoresinol（1）,cleomiscosin C（2）,cleomiscosin A（3）,3,4-dihydroxyphenyl aldehyde（4）,caffeic acid（5）,1-O-caffeoylquinic acid（6）,4-O-caffeoylquinic acid（7）and 1-O-caffeoylquinic acid methyl ester（8）.Conclusions All compounds are obtained from this genus for the first time.