TRX-1和STAT-3在结直肠癌组织中的表达及相关性研究

Protein expression of TRX-1 and STAT-3 in colorectal cancer and their correlation

目的检测人结直肠癌组织中TRX-1和STAT-3的表达,探讨二者之间的相关性。方法用免疫组织化学法分别检测60例结直肠癌和22例远癌正常大肠黏膜中TRX-1和STAT-3的表达情况,并结合临床资料,对其进行分析。结果 TRX-1和STAT-3均定位于细胞质和细胞核,TRX-1在结直肠癌与正常大肠黏膜组织阳性率分别为78.33%与31.82%(免疫组化染色平均评分分别为4.03±1.92和1.42±1.20),二者间差异有统计学意义(P<0.01);STAT-3在结直肠癌与正常大肠黏膜组织的阳性率分别为65.00%与27.27%(免疫组化染色平均评分分别为3.34±1.81和1.26±1.19),二者间差异有统计学意义(P<0.01)。TRX-1与STAT-3在低分化癌中比高分化者表达增强;二者的表达与淋巴结转移和TNM分级密切相关(P<0.05或P<0.01)。TRX-1和STAT-3在结直肠癌中的表达呈正相关(r=0.964,P<0.01)。结论 Trx-1和STAT-3在结直肠癌组织中过度表达,并与其发生发展有关,二者可能参与了结直肠癌恶化的调控。

Objective To investigate the expression of Trx-1 and STAT-3 in colorectal cancer and their relations. Methods The expression of TRX-1 and STAT-3 was detected using immunohistochemical SP method. Results In colorectal cancers with strong TRX1 and STAT-3 straining,the straining was seen in nucleus as well as in the cytoplasm. Of 60 cases of colorectal cancer,47（ 78. 33% ） showed positive staining of TRX-1（ immunohistochemical staining score 4. 03 ± 1. 92） ,while 7（ 31. 82% ） of 22 cases of normal mucosa showed positive staining of TRX-1（ immunohistochemical staining score 1. 42 ± 1. 20） . Similarly,39 cases（ 65. 00% ） showed positive staining of STAT-3（ immunohistochemical staining score 3. 34 ± 1. 81） in 60 cases of colorectal cancer,while only 6（ 27. 27% ） showed positive staining of STAT-3 in 22 cases of normal small intestinal mucosa（ immunohistochemical staining score 1. 26 ± 1. 19） . There was significant difference in TRX-1 and STAF3 expression between colorectal cancer mucosa and normal intestinal mucosa. The expression levels of TRX-1 and STAT-3 in low differentiated colorectal cancer were higher than that in high differentiated colorectal cancer（ P 0. 05 or P 0. 01） . Also,the expression of TRX-1 and STAT-3 was significantly correlated with lymph node metastasis and the Dukes’ stage（ P 0. 05 or P 0. 01） . TRX-1 expression was positively correlated with STAT-3 expression in colorectal cancer（ r = 0. 964,P 0. 01） . Conclusion The results indicate that the overexpression of TRX-1 and STAT-3 may be involved in the development of colorectal cancer. Both TRX-1 and STAT-3 may participate in the regulation of carcinogenesis,development and aggravation to colorectal cancer. Combined detection of TRX-1 and STAT-3 may be helpful to evaluate the infiltrative capability of colorectal cancer.