门静脉高压大鼠胃黏膜血管内皮生长因子的表达

Expression of increased vascular endothelial growth factor in gastric mucosa of portal hypertensive rats

目的探讨血管内皮生长因子(VEGF)在门静脉高压性胃病(PHG)大鼠胃黏膜的表达。方法将28只SD大鼠随机分为门脉高压胃病组(实验组)16只与对照组12只,应用八道生理记录仪测量门静脉压力(PVP),中性红清除率方法测量胃黏膜血流量(GMBF),应用免疫组织化学测定VEGF的表达。结果实验组PVP高于对照组(P<0.01);实验组GMBF增加(P<0.05);实验组VEGF阳性细胞明显多于对照组(P<0.05)。结论 VEGF在PHG大鼠中表达明显增高,VEGF可能参与门脉高压大鼠胃黏膜病变。

Objective To explore the expression of vascular endothelial growth factor （VEGF） in gastric mucosa of portal hypertensive and sham-operated rats.Methods 28 of male sprague dawley rats were randomly divided into two groups：portal hypertensive gastropathy （test group）of 16 and control group of 12.Fourteen days after a portal ligation or sham operation,the portal venous pressure was measured with physiology recorder,and the gastric mucosal blood flow volume was measured with the neutral red clearance method.The expression of VEGF in gastric mucosal specimens was immunohistochemically assessed.Results The portal pressure in the test group rats was significantly greater than that in the control group（P0.01）;Gastric mucosal blood flow in the test group was significantly greater than in the control group（P0.05）;The positive cells of VEGF increased obviously and level of VEGF protein in gastric organs was significantly higher in test group than that in control group（P0.05）.Conclusion The increased activity of VEGF may thus plays a role in gastric mucosal lesion of portal hypertensive rats.