肺炎支原体两种检测方法的比较

Comparison of two methods of mycoplasma pneumoniae-specific IgM antibody and fluorescent quantization PCR

目的比较肺炎支原体(MP)抗体检测法与荧光定量PCR法在肺炎支原体感染中的应用。方法对本院儿科181例下呼吸道感染的血液标本运用酶联免疫吸附试验(ELISA)法进行抗体检测(MP-IgM),同时运用PCR方法对其痰液标本进行检测(FQ-MP-DNA)。结果血抗体检测法与痰荧光定量PCR法比较差异无统计学意义,但后者对1岁以下的患者检出率更高。结论两种检测方法结合可取长补短,提高支原体感染的检出率。

Objective To compare the two methods of mycoplasma pneumoniae-specific IgM（MP-IgM） antibody detection and fluorescent quantization PCR in the treatment of infection of mycoplasma pneumoniae.Methods The method of ELISA was employed to detect MP-IgM antibody in the blood of 181 cases of respiratory infection,at the same time,the DNA of sputum of those patients was detected by means of fluorescent PCR.Results The two methods showed no significant difference in detection rate,while the latter had higher positive rate in children less than one year old.Conclusion The two methods have no difference in detection rate;it is a better way to use them concurrently,which is potential for a higher positive finding.