南宁市散发性急性胃肠炎病例中检出GⅠ.8型诺如病毒

Norovrius GenotypeⅠ.8 Being Detected from Sporadic Cases of Acute Gastroenteritis in Nanning Municipal

目的研究南宁市某医院散发性急性胃肠炎病例中,GⅠ.8型诺如病毒(Norovirus,NV)的基因特征。方法采用荧光定量逆转录-聚合酶链反应,对2007年1月～2008年12月南宁市某医院散发性急性胃肠炎病例标本检测基因Ⅰ型(GenogroupⅠ,GⅠ)NV核糖核酸,对阳性标本进行N/S区核苷酸序列测定,并构建系统进化树分析。结果在696份粪便标本中,检出3份GⅠ型NV核酸阳性。经核酸序列分析发现,其中一份[南宁(Nanning,NN)07230]与GⅠ.8型参考株(WUGⅠ/00/JP)的核苷酸序列同源性达94.5%;与2006～2007年日本、韩国公布的GⅠ.8型NV核苷酸序列同源性范围在96.7%～98.5%。NN07230在进化树中与GⅠ.8型病毒株属同一分支。结论在南宁市散发性急性胃肠炎病例中存在GⅠ.8型NV感染。

Objective To study the genic characteristic of Norovrius genotypeⅠ.8 from sporadic gastroenteritis of a hospital in Nanning Municipal.MethodsSpecimens of diarrhea patients from a hospital in Nanning Municipal between January 2007 and December 2008 were collected and detected for Noroviral genogroupⅠribonucleic acid（RNA） by real-time reverse transcription-polymerase chain reaction（real-time RT-PCR）.All positive specimens were subjected for sequencing analysis and constructed phylogenetic tree.ResultsNorovirus genogroupⅠRNA were detected in 3 of 696 stool specimens.The homology between one of the four strains（NN07230） and reference strain of GⅠ.8（WUGⅠ/00/JP） was 94.5%;and the homology between the NN07230 strain and strains circulating during 2006-2007 in Japan and Korea ranged from 96.7% to 98.5%.And they formed one cluster in the phylogenetic tree.ConclusionThe research showed that genotypeⅠ.8 infection exists in sporadic gastroenteritis in Nanning City.