矮溲疏组培快繁中的茎芽增殖与生根培养研究

Studies on Micro-Shoot Multiplication and Rooting During in vitro Propagation of Deutzia hybrida 'Boule'

[目的]探讨矮溲疏试管苗的茎芽增殖和生根培养技术。[方法]分别研究培养基中不同的大量元素浓度（1/4MS、1/2MS和MS）、IBA浓度（0~0.8 mg/L）和蔗糖浓度（5、10、15和30 g/L）对矮溲疏试管苗茎芽增殖和生根培养的影响。[结果]大量元素浓度为1/2MS处理的茎芽增殖系数可达3.9,与MS处理差异不明显,但在0.05水平显著高于1/4MS处理;0~0.8 mg/L IBA对矮溲疏试管苗的茎芽增殖无明显作用;蔗糖浓度为5 g/L处理的茎芽增殖系数为3.8,与10和30 g/L处理无明显差异,但在0.05水平显著高于15 g/L处理。上述处理的试管苗生根率均为100%。以土取代琼脂作培养基支撑物进行矮溲疏试管苗的生根培养,生根率可达100%,根茎比大于琼脂支撑培养对照,根毛也长于对照。[结论]该研究为矮溲疏组织培养快繁技术体系的建立奠定了基础。

[Objective] This study aimed at exploring the technique of micro-shoot multiplication and rooting of Deutzia hybrida ＇Boule＇ plantlet in vitro.[Method] The different macro-element concentration（1/4MS,1/2MS or MS）,IBA concentration（0~0.8 mg/L） and sucrose concentration（5,10,15 or 30 g/L） were used respectively for micro-shoot multiplication and rooting of Deutzia hybrida ＇Boule＇ plantlet.[Result] The micro-shoot multiplication coefficient of 1/2MS treatment was 3.9 which had no obvious difference with MS treatment,but was obvious higher than that of 1/4 MS treatment.The IBA concentrations from 0 to 0.8 m g/L had no obvious effect on micro-shoot multiplication of Deutzia hybrida ＇Boule＇.The micro-shoot multiplication coefficient of 5 g/L sucrose treatment was 3.8 which had no obvious difference with both 10 and 30 g/L sucrose treatments,but was obvious higher than that of 15 g/L sucrose treatment.The rooting rate of the plantlet in all the treatment above was 100%.When cultured in medium supported by soil instead of agar,the root rate was 100%.The root/shoot ratio of Deutzia hybrida ＇Boule＇ plantlet cultured in soil supporting medium was obvious higher than that of the plantlet in agar medium and the root hairs of Deutzia hybrida ＇Boule＇ plantlet cultured in soil supporting medium was longer than that of the plantlet in agar medium.[Conclusion] This study provided the basis for the foundation of Deutzia hybrida ＇Boule＇ micro-propagation technique system.