秋水仙素诱导阔叶风铃草多倍体研究

Progress of Polyploidy Induction by Colchicine in Campanula medium L.

[目的]探讨秋水仙素诱导阔叶风铃草多倍体的最佳方法。[方法]以阔叶风铃草成熟种子为试材,研究不同浓度秋水仙素、处理时间和处理方法对风铃草多倍体诱导的影响。[结果]幼苗用质量分数为0.2%的秋水仙素溶液浸泡16 h后,其多倍体的诱导率为16.0%,高于其他处理方法。[结论]秋水仙素处理浓度和时间对阔叶风铃草的变异率有明显影响。

[Objective] To explore the optimum method of polyploidy induction by colchicine in Campanula medium L.[Method] The mature seeds of Campanula medium L.were used as materials,and the effects of colchicine concentration,treatment time and methods on the polyploidy induction ratios were studied.[Result] The results showed that the optimum method of polyploid induction was soaking seeds in 0.2% colchicum for 16 h,and the highest induction ratio was about 16.0%.[Conclusion] The adequate treatment concentration and duration can significantly enhance the mutational rate of Campanula medium L.