大孔吸附树脂纯化羟基红花黄色素A的研究

Studies on Purification of Hydroxy Safflower Yellow A from Carthamus tinctorius L.by Macroporus Resins

[目的]探讨大孔吸附树脂对红花提取物的最佳纯化条件及纯化效果,为羟基红花黄色素A的工业化高效生产奠定基础。[方法]以羟基红花黄色素A（HSYA）作为考察指标,通过羟基红花黄色素A在6种商品化树脂上的吸附量和解吸率筛选树脂的种类,以收率和纯度为指标考察上样液的吸附流速、pH值及浓度,洗脱液的浓度、流速等纯化条件。[结果]采用HPD-400作为吸附树脂,50%乙醇作为洗脱液,pH值为3的红花提取液,上样吸附流速为6 BV/h,洗脱流速为8~10 BV/h时纯化效果较好,在该条件下HSYA的收率为73.8%,纯度为35.7%。[结论]大孔吸附树脂法优化纯化条件后,提高了羟基红花黄色素A的纯化效果,这为羟基红花黄色素A的工业化高效生产提供了理论依据。

[Objective]The research aimed at obtaining the optimum conditions for the isolation and purification of hydroxy safflower yellow A（HSYA）from carthamus tinctorius L.extracts using macroporous resins and providing basis for the effective industrial production of HSYA.[Method]Resins were screened through the determination of adsorption ratio and desorption ratio of Carthamus tinctorius L.The yield and purity of HSYA were used as indices to investigate the purification conditions,including the flow speed of adsorption,the flow speed of cleaning agent,the kind and the flow speed of the elution agent,the ratio of diameter to height of resin column etc.[Result]HPD400 was selected as the adsorbed resin,50% ethanol was chosed as the elution agent,the flow speed of adsorption was 6 BV/h,and the flow speed of elution was 8-10 BV/h.Under the purification condition,the yield of hydroxy safflower yellow A was 73.8% and the purity was 35.7%.[Conclusion]The purification efficiency of HSYA is promoted greatly by purifying,which will provide the theoretical evidence for effective industrial production of Carthamus tinctorius L.